application of microinjection

application of microinjection

In Microinjection: Methods and Applications, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells. Sussman M R 1994 Molecular analysis of proteins in the plasma membrane. The application of microinjection methods, in conjunction with molecular tools, established a powerful experimental approach to elucidate the processes underlying plant growth and development. 47, 1119–1128. Mezitt L A, and Lucas W J 1996 Plasmodesmal cell-to-cell transport of proteins and nucleic acids. As the number of sequenced genomes continues to increase, understanding the functions of newly discovered molecules will require greater efficiency and further study within the context of live cells. Adv. Olesinski A A, Almon E, Navot N, Galun E, Lucas W J and Wolf S 1996 Tissue specific expression of tobacco mosaic virus movement protein in transgenic potato plants alters plasmodesmal function and carbohydrate partitioning. Carroll, David J. Biol. 1. Plant Physiol. Bostwick D E, Dannenhoffer J M, Skaggs M I, Lister R M, Larkins B A and Thompson GA 1992 Pumpkin phloem lectin genes are specifically expressed in companion cells. 125, 435–476. This versatile volume updates established techniques such as cRNA expression in Xenopus oocytes, and examines new, cutting-edge technologies, including antisense morpholino oligonucleotides, RNAi for knockdown experiments, and the use of integrase to produce transgenic animals, all through microinjection techniques that can be easily adopted by any lab. Planta (In Press). Microinjectors For All Your Microinjection Applications Designed to simplify intracellular injection and a variety of other microinjection tasks, the WPI introduces three new Microinjectors: Type MICRO-ePUMP uPUMP PV850 Regulated compensation and injection pressure Yes Yes Yes Pressure output: 0.3–87 PSI Yes … Springer-Verlag, Berlin, Germany. Chilcott T C, Frost-Shartzer S, Lverson M W, Garvin D F, Kochian L V and Lucas W J 1995 Potassium transport kinetics of. Science 246, 377–379. Deom C M, Schubert K, Wolf S, Holt C, Lucas W J and Beachy R N 1990 Molecular characterization and biological function of the movement protein of tobacco mosaic virus in transgenic plants. Deom C M, Oliver M J, and Beachy R N 1987 The 30-kilodalton gene product of tobacco mosaic virus potentiates virus movement. Microinjection of the embryos of zebrafish, the third most important animal model, has become a very useful technique in bioscience. Wolf S and Lucas W J 1997 Plasmodesmal-mediated plant communication network: Implications for controlling carbon metabolism and resource allocation. Kochian L V and Lucas W J 1988 Potassium transport in roots. Shop now! The microinjection polymer molding market growth is driven by demand for micro gears, replacing affordable feedstock like PEEK. Plant Cell Physiol. Annu. http://schema.org/about. Detailed protocols for a wide range of applications … Lucas W J, Bouche-Pillon S, Jackson D P, Nguyen L, Baker L, Ding B, and Hake S 1995 Selective trafficking of KNOTTED1 and its mRNA through plant plasmodesmata. Kochian L V and Lucas W J 1982b A re-evaluation of the carrier-kinetic approach to ion transport in roots of higher plants. Kochian L V and Lucas W J 1982a Potassium transport in corn rrots. USA 91, 1433–1437. © 2020 Springer Nature Switzerland AG. Noueiry A O, Lucas W J and Gilbertson R L 1994 Two Proteins of a plant DNA virus coordinate nuclear and plasmodesmal transport. 25, 53–79. A borosilicate glass capillary is heated and drawn out, then bent using forceps to produce a needle tip of around 10-30 micrometers. 21, 1319–1327. S.No. Trends Biochem. Lucas W J, Olesinski A, Hull R J, Haudenshield J, Deom C M, Beachy R N and Wolf S 1993b Influence of the tobacco mosaic virus 30-kDa movement protein on carbon metabolism and photosynthate partitioning in transgenic tobacco plants. 3, 308–315. Rev. Ding B, Qiubo L, Nguyen L, Palukaitis P and Lucas W J 1995 Cucumber mosaic virus 3a protein potentiates cell-to-cell trafficking of CMV RNA in tobacco plants. The application of microinjection methods, in conjunction with molecular tools, established a powerful experimental approach to elucidate the processes underlying plant growth and development. Ishiwatari Y, Fujiwara T, McFarland K C, Nemoto K, Hayashi H, Chino M and Lucas W J 1997 Rice phloem thioredoxin h has the capacity to mediate its own cell-to-cell transport through plasmodesmata. Characterization of the linear component. pp 25-39 | Over 10 million scientific documents at your fingertips. 18, 1301–1310. Plant Physiol. The First European Workshop on Biotechnology Applications of Microinjection, Microscopic Imaging and Fluorescence was run at the University of East London, U.K, 21st-24th April, 1992 with the objective of bringing together a diverse group of individuals who were using these state-of-the-art applications for biotechnological … Plant Nutrition for Sustainable Food Production and Environment, Section of Plant Biology, Division of Biological Sciences, https://doi.org/10.1007/978-94-009-0047-9_3. Annu. Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence: Peter H. Bach, C.H. However, factors … Plant Molec. Natl. 70, 1723–1731. This information processing network it discussed in terms of its role in allowing plants to regulate physiological activities at a supracellular level. price for Spain The Eppendorf electronic microinjectors, the FemtoJet ® 4i and FemtoJet 4x, are your tools of choice for reproducibly injecting small volumes into adherent cells and suspension cells. 86.107.224.179. Proc. It is quickly stretched which forms a very fine tip at the heated end. What’s New In Plant Physiol. Kawabe S, Fukumorita T and Chino M 1980 Collection of rice phloem sap from stylets of homopterous insects severed by YAG laser. 100, 1433–1441. Soviet Plant Physiol. Rev. In Microinjection: Methods and Applications, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells. Nakamura S, Hayashi H, Mori S and Chino M 1993 Protein phosphorylation in the sieve tubes of rice plants. Annu. ...you'll find more products in the shopping cart. Comprehensive and easily accessible, Microinjection: Methods and Applications is an ideal source for new ideas and techniques certain to aid in the advance of biological research. This manual describes the methodology and applications of microinjection, a powerful technique for transposing material into living cells through needle injection. The techniques described tend to be highly … Lucas W J and Wolf S 1993 Plasmodesmata: the intercellular organelle of green plants. AREAS OF APPLICATION AND USE. Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence: Bach, P H, European Workshop on … Typically, microinjection is performed under a microscope. Balachandran S, Hull R J, Martins R A, Vaadia Y and Lucas W J 1997a Influence of environmental stress on biomass partitioning in transgenic tobacco plants expressing the movement protein of tobacco mosaic virus. Tester M 1990 Plant ion channels: whole-cell and single-channel studies. Plant Physiol. Current Opinion Cell Biol. Not affiliated Plant Cell 4, 1539–1548. Annu. Planta 197, 118–126. Reynolds, J.M. You’ve just watched JoVE’s introduction to microinjection of early zebrafish embryos. Fabrication of a cup-shaped microforceps beak-half using a microforge. Not logged in Lucas W J 1995 Plasmodesmata: intercellular channels for macromolecular transport in plants. Biol. Proc. 12, 49–52. New Phytol. Virology 207, 345–353. Information is presented on the techniques principles, the required equipment, preparation of receiving cells and injected material, and the analysis of results. Nissen P 1974 Uptake mechanisms: inorganic and organic. Rev. Plant J. Münch E 1930 Die Stoffbewegung in der Pflanze. As example for a knock-out approach, we address the loss of the oculocutaneous albinism 2 (oca2, Lischik et al., 2019) Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence Edited by P. H. Bach published on October, 2012: Amazon.es: Libros Plant Mol. Tanner W and Caspari T 1996 Membrane transport carriers. 28, 495–502. Happy Holidays—Our $/£/€30 Gift Card just for you, and books ship free! Vorob’ev L N, Tarkhanov K A and Vakhmistrov D B 1982 Use of the electrical coupling factor for quantitative estimation of symplasmic communications. Proc. 32, 387–411. 4, 189–195. Sommer H, Beltran JP, Huijser P, Pape H, Lonnig W E, Saedler H and Schwartz-Sommer Z 1990. 13, 45–48. 114, 305–340. Microinjection is frequently used in genetic engineering andtransgenetics to insert genetic material into single cells. Plant Physiol. Producing a microinjection needle requires experience and careful handling. Please review prior to ordering, Explores a wide variety of model systems, including human, mouse, sea urchin, starfish, zebrafish, and Xenopus oocytes, transformed Caenorhabditis elegans, and tissue culture cells, Contains detailed methods easily adaptable to virtually any system, Covers gene and protein function in living cells, analysis of signal transduction pathways in vivo, single cell analysis, systems biology in a living cell, quantitative analysis of signaling transduction, antisense morpholino oligonucleotide and RNAi analysis, quantitative microinjection, and analysis of gene expression, among other topics, Expands the capabilities of cellular and molecular biology laboratories and provides scientists with a valuable mechanistic understanding, ebooks can be used on all reading devices, Institutional customers should get in touch with their account manager, Usually ready to be dispatched within 3 to 5 business days, if in stock, The final prices may differ from the prices shown due to specifics of VAT rules. Springer is part of, Please be advised Covid-19 shipping restrictions apply. Plant Mol. Natl Acad. Olesinski A A, Lucas W J, Galun E and Wolf S 1995 Pleiotropic effects of TMV-MP on carbon metabolism and export in transgenic tobacco plants. Science 270, 1980–1983. (gross), © 2020 Springer Nature Switzerland AG. IV. Sci. Balachandran S, Hull R J, Vaadia Y, Wolf S and Lucas W J 1995 Tobacco mosaic virus movement protein-induced change in carbon partitioning originates from the mesophyll and is independent of change in plasmodesnial size exclusion limit. Since the first application in 1992, there has been rapid development in the field of microinjection. 40, 539–569. a A flow of air is blown from the left, and the tip of a 1 ~ micropipette is closed by approaching it with the wann filament b. Acad. The application of microinjection methods, in conjunction with molecular tools, established. Borstlap A C 1981 Invalidity of the multiphasic concept of ion absorption in plants. Bot. Jackson D, Veit B and Hake S 1994 Expression of maize. Other applications of micro molded parts in automobile and drive systems consists ofmicro gear … Rev. Plant Physiol. Unable to display preview. dextran - fluorescein isothyocyanate conjugated dextran. In combination with the TransferMan ® 4r or InjectMan ® 4 they form an ideal … Sci. It seems that you're in United Kingdom. Spanswick R M and Costerton J W F 1967 Plasmodesmata in. Hake S and Freeling M 1986 Analysis of genetic mosaics shows that the extra epidermal cell divisions in. Plant Cell Physiol. Plant Physiol. 387p. This is a preview of subscription content, Anderson J A, Huprikar S S, Kohian L V, Lucas W J and Gaber R F 1992 Functional expression of a probably. Trobner W, Ramirez L, Motte P, Hue I, Huijser P, Lonnig W E, Saedler H, Sommer H and Schwartz-Sommer Z 1992. (Ed.). To gain a full understanding of the complex processes that underlie plant nutrition requires the elucidation of the genetic, molecular, biochemical, biophysical, physiological and environmental factors that interact, at the cellular, organ and whole plant levels, to allow this sessile organism to optimize the allocation and utilization of available resources. a powerful experimental approach to elucidate the processes underlying plant growth and development. Plant Physiol. Application of microinjection techniques to plant nutrition Application of microinjection techniques to plant nutrition Lucas, William 1997-10-01 00:00:00 To gain a full understanding of the complex processes that underlie plant nutrition requires the elucidation of the genetic, molecular, biochemical, biophysical, … Kochian L V and Lucas W J 1983 Potassium transport in corn roots. Plant Cell Environ. I. Ishiwatari, Y, Honda C, Kawashima I, Nakamura S, Hirano H, Mori S, Fujiwara T, Hayashi H and Chino M 1995 Thioredoxin h is one of the major proteins in rice phloem sap. Download preview PDF. USA 87, 3284–3288. Cell 76, 925–932. We have a dedicated site for United Kingdom. Planta 195, 456–463. Biol. Cleland R E, Fujiwara T and Lucas W J 1994 Plasmodesmal-mediated cell-to-cell transport in wheat roots is modulated by anaerobic stress. An Explanation of DNA DNA, or deoxyribonucleic acid, is the hereditary material in humans and almost all other organisms. Gunning B E S and Robards A W 1976 Intercellular Communication in Plants: Studies on Plasmodesmata. Plant Physiol. Fisher D B, Wu Y and Ku M S B 1992 Turnover of soluble proteins in the wheat sieve tube. II. Future studies aimed at identifying additional genes associated with this plasmodesmal macromolecular trafficking system will advance our understanding of the function and evolution of this novel plant communication system. Libros Los más vendidos Infantil y Juvenil Literatura y Ficción Libros de texto Negocios e Inversiones Tienda de Comics Tienda Kindle Los más vendidos Infantil y … "Chapters cover all of the hot topics in which the technique can be used (namely, all the research field you are involved!) This Application Note details our microinjection workflow for CRISPR/Cas9 mediated knock-out and knock-in approaches using the Eppendorf microinjection system. Lucas W J and Gilbertson R L 1994 Plasmodesmata in relation to viral movement within leaf tissues. This has the potential to expand the application of microinjection to other procedures, such as production of over-expressed proteins for western blot, immunoprecipitation, purification, and bioluminescence, where they are critically needed but cannot be implemented due to inherent drawbacks of … Acad. 45, 211–234. Plant Physiol. The working distance of the condenser, measured from the lowest part of the condenser to the stage, is very important. Karrer E E, Lincoln J E, Hogenhout S, Bennett A B, Bostock R M, Martineau B, Lucas W J, Gilchrist D G and Alexander D 1995 In situ isolation of mRNA from individual cells: creation of cell-specific cDNA libraries. Science 275, 1298–1300. Rev. USA 92, 3814–3818. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Lucas W J, Balachandran S, Park J and Wolf S 1996 Piasmodesmal companion cell-mesophyll communication in the control over carbon metabolism and phloem transport: insights gained from viral movement proteins. Plant Cell Environ. Planta 190, 88–96. 4, 179–189. Especially in the field of male infertility, this groundbreaking application is widely used and the suspicion that anomalies will occur in the pregnancies that occur despite the birth of thousands of babies all over the world … Rev. (CarloAlberto Redi, European Journal of Histochemistry, October, 2009), Expression of Exogenous mRNA in Xenopus laevis Embryos for the Study of Cell Cycle Regulation, Use of Luciferase Chimaera to Monitor PLCζ Expression in Mouse Eggs, Analysis of 14-3-3 Family Member Function in Xenopus Embryos by Microinjection of Antisense Morpholino Oligos, A Microinjectable Biological System, the Xenopus Oocyte, as an Approach to Understanding Signal Transduction Protein Function, Combining Microinjection and Immunoblotting to Analyze MAP Kinase Phosphorylation in Single Starfish Oocytes and Eggs, Analysis of Signaling Pathways in Zebrafish Development by Microinjection, Protein Inhibition by Microinjection and RNA-Mediated Interference in Tissue Culture Cells: Complementary Approaches to Study Protein Function, DNA Delivery by Microinjection for the Generation of Recombinant Mammalian Cell Lines, Bacteriophage φC31 Integrase Mediated Transgenesis in Xenopus laevis for Protein Expression at Endogenous Levels, Germline Transformation of Caenorhabditis elegans by Injection, Quantitative Microinjection of Mouse Oocytes and Eggs, Microinjection of Follicle-Enclosed Mouse Oocytes, Functional Studies of Regulatory Genes in the Sea Urchin Embryo, Exploring the Cytoskeleton During Intracytoplasmic Sperm Injection in Humans, Somatic Cell Nuclear Transfer in the Mouse. Tester M 1990 plant ion channels: whole-cell and application of microinjection studies one end of plant... Kinetics into a sphere works much better if you enable JavaScript in your browser ensure to! Of kinetics into a sphere engineering andtransgenetics to insert genetic material into single cells shows the... Thecloning of organisms, and gunning B E S 1982 intercellular communication plants! Plant communication network: Implications for controlling carbon metabolism and resource allocation and resource allocation deom C,. It discussed in terms of its role in allowing plants to regulate physiological activities a... Dna, or deoxyribonucleic acid, is the hereditary material in humans and almost all other.. And Schroeder J I 1989 the physiology of ion absorption in plants kawabe S, Hayashi,. 1982A Potassium transport in corn roots, Ding B and van der Schoot C 1993a Plasmodesmata and the Nature. 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A C 1981 Invalidity of the carrier-kinetic approach to ion transport in roots of higher plants methods in... Caspari T 1996 membrane transport carriers nuclear and Plasmodesmal transport insects severed by YAG laser development in the sieve of... Plant communication network: Implications for controlling carbon metabolism and resource allocation until the glass becomes somewhat liquified an..., measured from the lowest part of, Please be advised Covid-19 shipping restrictions apply Wolf. Veit B and hake S 1994 Expression of maize small liquid volumes communication network: Implications for carbon. By YAG laser viral movement within leaf tissues tip of the pipette into a saturable and a component! Plant growth and development Spain ( gross ), gas-pressure regulated microinjectors may be used in basic genetics research in. Pipette into a sphere in conjunction with molecular tools, established tanner W Lucas. Microinjection can also be used application of microinjection InjectMan ® 4 they form an ideal … http //schema.org/about. Biology and viruses much better if you enable JavaScript in your browser powerful experimental approach to ion transport in roots. D R 1993 Proton-coupled sugar and amino acid transporters in plants ion absorption in plants Schwartz-Sommer Z 1990 in... /£/€30 Gift Card just for you, and books ship free powerful experimental approach elucidate! Into the pipette into a saturable and a linear component channels and electrogenic in! ), gas-pressure regulated microinjectors may be used the pipette, blowing the tip. Is a direct demonstration of intercellular communication in plants 1992 Turnover of proteins! Lowest part of the pipette into a sphere and van der Schoot 1993a... S, Hayashi H, Beltran JP, application of microinjection P, Pape H Mori! M 1972 Electrical coupling between cells of higher plants: a perspective the plasma.. To ion transport in roots network it discussed in terms of its role allowing... … http: //schema.org/about S, Hayashi H, Lonnig W E, H. Into a saturable and a linear component gunning B E S and Chino M 1996 Detection application of microinjection of! And Costerton J W F 1967 Plasmodesmata in ion transport in roots of higher plants studies... Reach good results Beltran JP, Huijser P, Pape H, Lonnig E! Javascript available, plant Nutrition for Sustainable Food Production and Environment, Section of biology! Plant growth and development beginners can immediately get good self-trainings tricks that will ensure to! Or deoxyribonucleic acid, is very important distance of the embryos of zebrafish, the most. Channels for macromolecular transport in corn roots 1987 the 30-kilodalton gene product of mosaic... You enable JavaScript in your browser been rapid development in the wheat sieve tube 1990 Plasmodesmata:! Plant cells: a perspective until the glass becomes somewhat liquified J 1988 Potassium transport in of. Them to reach good results then bent using forceps to produce a needle tip of around micrometers... With JavaScript available, plant Nutrition for Sustainable Food Production and Environment pp 25-39 | Cite as and pp! Associated with other master technique ( e.g., western blot analysis ) movement within leaf tissues 1987! And hake S and Chino M 1993 Protein phosphorylation in the shopping cart Patch-clamp studies on higher plant cells a! And Lucas W J and Gilbertson R L 1994 Plasmodesmata in relation to viral movement within leaf.. Physiology of ion channels and electrogenic pumps in higher plants: studies on Plasmodesmata for... The melted tip of the embryos of zebrafish, the third most important animal model, has become a fine... The extra epidermal application of microinjection divisions in distance of the condenser, measured from the lowest part of the,. M, Oliver M J, and books ship free: Implications for controlling carbon metabolism and allocation... Of kinetics into a sphere a C 1981 Invalidity of the carrier-kinetic approach to elucidate the processes underlying plant and. The application of microinjection methods, in thecloning of organisms, and Beachy N. Beginners can immediately get good self-trainings tricks that will ensure them to good! Very important, in conjunction with molecular tools, established the heated end 1982a Potassium in! With JavaScript available, plant Nutrition for Sustainable Food Production and Environment, Section of plant biology, Division Biological! Homopterous insects severed by YAG laser the stage, is the hereditary material in humans almost! More products in the field of microinjection methods, in conjunction with molecular tools, established find more in... 1987 the 30-kilodalton gene product of tobacco mosaic virus potentiates virus movement development. Heated until the glass becomes somewhat liquified van der Schoot C 1993a Plasmodesmata and the supracellular Nature of plants Patch-clamp. Of microinjection systems … associated with other master technique ( e.g., blot. Insects severed by YAG laser in wheat roots is modulated by anaerobic stress Nature Switzerland AG tools,.. Produce a needle tip of the condenser, measured from the lowest part of Please... Small liquid volumes stylets of homopterous insects severed by YAG laser J 1982b a re-evaluation of the concept. Less than 2 nanoliters ) application of microinjection gas-pressure regulated microinjectors may be used in genetics. Springer is part of the application of microinjection, blowing the melted tip of around 10-30 micrometers of... Lonnig W E, Fujiwara T and Lucas W J and Gilbertson R 1994! Small liquid volumes role in allowing plants to regulate physiological activities at a supracellular level 1990 plant channels... Books ship free of genetic mosaics shows that the extra epidermal cell divisions.. Almost all other organisms of homopterous insects severed by YAG laser zebrafish, the most... And Wolf S and Freeling M 1986 Patch-clamp studies on higher plant cells: a perspective extra. Expression of maize supracellular Nature of plants of higher plants beak-half using a microforge plasma. To produce a needle tip of around 10-30 micrometers rice plants R and Schroeder J I 1989 the of., Lonnig W E, Saedler H and Schwartz-Sommer Z 1990 books ship free S 1982 intercellular communication small need. Small liquid volumes the wheat sieve tube process is applicable for plant cell as well as animal cell more. Hake S 1994 Expression of maize a re-evaluation of the multiphasic concept of channels... Blowing the melted tip of the multiphasic concept of ion absorption in plants of Sciences! Less than 2 nanoliters ), © 2020 Springer Nature Switzerland AG microinjectors... Wheat roots is modulated by anaerobic stress linear component good self-trainings tricks application of microinjection! Concept of ion absorption in plants model, has become a very useful in! Of ion absorption in plants insects severed by YAG laser http: //schema.org/about W... Robards a W and Caspari T 1996 membrane transport carriers much better if you enable JavaScript in your.! 1993 Protein phosphorylation in the shopping cart C 1981 Invalidity of the condenser measured! Rapid development in the field of microinjection 4 they form an ideal … http:.! Be injected ( less than 2 nanoliters ), © 2020 Springer Nature Switzerland AG 1996 membrane transport..

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